NAD+ 500mg

Description

Nicotinamide Adenine Dinucleotide (NAD+) is a coenzyme found in all living cells that plays a central role in cellular metabolism, energy production, and DNA repair. As one of the most fundamental molecules in biochemistry, NAD+ participates in over 500 enzymatic reactions, serving as a critical electron carrier in the mitochondrial electron transport chain and as a substrate for key regulatory enzymes including sirtuins, PARPs (poly-ADP-ribose polymerases), and CD38.

Research into NAD+ biology has accelerated dramatically following the discovery that NAD+ levels decline significantly with age. Studies in multiple model organisms have demonstrated that this age-related NAD+ decline correlates with mitochondrial dysfunction, impaired DNA repair capacity, and the onset of numerous age-associated pathologies. In landmark studies by Sinclair and colleagues at Harvard Medical School, restoration of NAD+ levels in aged mice through precursor supplementation resulted in dramatic improvements in mitochondrial function, with aged tissue resembling that of much younger animals within one week of treatment.

The sirtuin pathway represents one of the most intensively studied NAD+-dependent signaling cascades. Sirtuins (SIRT1-7) are NAD+-dependent deacylases that regulate numerous cellular processes including gene expression, stress response, metabolism, and circadian rhythms. SIRT1 and SIRT3 in particular have been identified as key mediators of the beneficial effects of caloric restriction, the only intervention consistently shown to extend lifespan across species. By maintaining adequate NAD+ pools, sirtuin activity is sustained, supporting cellular homeostasis and stress resilience.

PARP enzymes represent another critical NAD+-dependent system. PARPs consume NAD+ during DNA repair processes, and chronic DNA damage — which accumulates with age — progressively depletes cellular NAD+ reserves. This creates a vicious cycle where declining NAD+ impairs both PARP-mediated DNA repair and sirtuin-mediated protective gene regulation. Research has shown that NAD+ repletion can break this cycle, restoring DNA repair capacity and reducing accumulated DNA damage in aged tissues.

NAD+ has also been investigated in the context of neurodegeneration, cardiovascular function, and immune regulation. In preclinical studies, NAD+ augmentation protected against axonal degeneration, improved cardiac function in models of heart failure, and enhanced the function of aged immune cells. Multiple clinical trials are currently underway investigating NAD+ precursors for conditions ranging from age-related cognitive decline to metabolic syndrome. This product is supplied as a white lyophilized powder at 500mg per vial, exceeding 98% purity by HPLC analysis.

Composition

• Compound: NAD+ (Nicotinamide Adenine Dinucleotide)
• Molecular Formula: C21H27N7O14P2
• Molecular Weight: 663.43 g/mol
• CAS Number: 53-84-9
• Purity: >98% (HPLC)
• Appearance: White to off-white lyophilized powder
• Quantity: 500mg per vial

Storage

• Store lyophilized powder at -20°C for long-term storage (up to 24 months).
• For short-term storage (up to 2 weeks), refrigerate at 2-8°C.
• After reconstitution, store at 2-8°C and use within 14 days.
• NAD+ is sensitive to light and heat — protect from both at all times.
• Avoid repeated freeze-thaw cycles.

Certificate of Analysis

Every batch of NAD+ sold by Pepspan undergoes rigorous third-party testing. Our Certificate of Analysis (COA) includes HPLC purity analysis, mass spectrometry confirmation, endotoxin testing, and sterility verification. COA documentation is available upon request for any batch number. All testing is performed by independent European laboratories.

Current Batch: Pending first shipment

Every batch of NAD+ 500mg sold by Pepspan is independently tested by Janoshik Analytical (Prague, Czech Republic). HPLC purity analysis, mass spectrometry, and amino acid sequencing are performed for each shipment. Reports are batch-specific — not generic.

Research Disclaimer

This product is intended for laboratory research use only. It is not a drug, food, cosmetic, or dietary supplement. NAD+ is not approved for human or veterinary use by any regulatory agency. It must not be administered to humans or animals outside of approved clinical trials. By purchasing this product, you confirm that you are a qualified researcher and that this product will be used exclusively for legitimate scientific research purposes. Pepspan assumes no liability for any misuse of this product.